development of a method for dna extraction from oil palm leaves in Johannesburg

Academia.eduDNA Extraction from Mature Oil Palm

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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Development of a method for DNA

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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Plant ArchivesCOMPARISON BETWEEN THREE DIFFERENT METHODS

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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A SIMPLE AND RAPID PROTOCOL FOR ISOLATION

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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PALMOILISthe need (mohd basri) Malaysian Palm Oil Board

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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Quality control on oil palm RNA samples for efficient

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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جامعة البصرةDEVELOPMENT, IMPLEMENTATION OF AN EFFICIENT

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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DEVELOPMENT, IMPLEMENTATION OF AN EFFICIENT

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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Academia.eduA simple method for DNA extraction

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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DEVELOPMENT, IMPLEMENTATION OF AN EFFICIENT

development of a method for dna extraction from oil palm leaves in Johannesburg
                                               
                                               
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  • How to extract DNA from oil palm leaves without liquid nitrogen?
  • Progress would require an efficient, low cost and high-throughput deoxyribonucleic acid (DNA) extraction method adapted for oil palm. A method for genomic DNA extraction from oil palm leaves that is simple and cost-effective was developed without the use of liquid nitrogen. A 260/280 ratios between 1.987 and 2.078 were obtained.
  • How to extract high-molecular-weight DNA from mature palm leaves?
  • A method is presented for the rapid isolation of high-molecular-weight DNA from mature leaves of date palm (Phoenix dactylifera L.), using a CTAB-based buffer. The method yields up to 800 μg of DNA from 1 g of leaf tissues. The procedure was also suitable for DNA extraction from callus or buds from tissue culture.
  • How is DNA extracted from palm trees?
  • Using the developed protocol, DNA was isolated from 10 palm trees comprising four pisifera, one tenera (T) and five dura palms. Eight microliters (8 µl) of each sample were loaded on a 0.8% agarose gel and the gel was stained with ethidium bromide. Total DNA from oil palm leaves of 93 trees extracted with the protocol.
  • What are the components of DNA extraction?
  • The components of the DNA extraction buer used a previous protocol used for mature leaves of oil palm [ 6]. Mannitol, of Tris-HCl, EDTA and SDS are also dierent. The method reported [ 7, 8]. Liquid nitrogen, which is not always easy to obtain in most third Billotte et al. (Table 1). The cost of DNA extraction per sample
  • Can leaf sampling be used for DNA extraction?
  • Regarding the leaf sampling for (spear) leaves can also be used for DNA extraction. concentration of the extracted DNA. Here, we determined the eect of metric absorbance of the extracted nucleic acids. The method reported as visible in Figure 1. The treatment of the nucleic acids with RNase was omitted for two reasons.
  • Which CTAB extraction method is used for AFLP study?
  • Of these, method K, a modified CTAB extraction method with optimized extraction protocol and optimum chemical components in the buffers, was selected for DNA extraction for the AFLP study using mature oil palm leaves. The DNA yield from each gramme of leaf tissue was consistently above 200 mg.